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母种培养基,一般都用琼脂作凝固剂,但在山区农村难以买到琼脂。为此,我进行了大米磨浆代琼脂试验,取得了较好效果,现将具体做法简介如下:将大米用温水浸泡12小时,加水量为超过米面2～3厘米。浸后用磨磨成浓浆(反复磨2～3次),然后加适量葡萄糖、磷酸二氢钾,拌匀后分装试管,装量为试管长的1/5,然后取一个平底茶盆,盆内放一条小木棒把试管搁在木棒上摆成斜面,置洁净的铁锅蒸架上(离水面2～3厘米),用猛火蒸至冒气后维持30～45分钟起锅,此时培养基已凝结成半透明的斜面。如果试管内有水珠,要倒掉水后再塞棉塞、包扎牛皮纸,进行高压灭菌。
Maternal medium, generally used agar as coagulant, but difficult to buy agar in rural areas. To this end, I conducted a rice refining agar test, and achieved good results, the specific practices are as follows: The rice soaked with warm water for 12 hours, add water to more than 2 to 3 cm rice. After immersion grinding with a thick slurry (repeated grinding 2 to 3 times), and then add the appropriate amount of glucose, potassium dihydrogen phosphate, mix well after dispensing tubes, loading capacity of test tube length 1/5, and then take a flat-bottomed tea pot, Place a small wooden stick put the test tube in a wooden stick placed on a bevel, set a clean iron steaming rack (2 to 3 cm from the surface of the water), with a fierce fire to steaming to maintain 30-60 minutes after the gas, The culture medium has condensed into a translucent bevel. If there is a drop of water in the test tube, pour the tampon and plug the kraft paper and autoclave.