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目的探索榆黄喷雾剂中盐酸小檗碱的薄层鉴别方法,为制订榆黄喷雾剂质量标准提供参考。方法照薄层色谱法试验,将各种试样分别点于同一硅胶G薄层板上,以乙酸乙酯-丁酮-甲酸-水(10∶6∶1∶1)为展开剂,365 nm紫外灯下检视。观察供试品色谱中,在与对照品色谱相应位置上的荧光斑点。结果供试品、黄柏对照药材溶液色谱中,在与对照品色谱相应的位置上,显相同颜色的荧光斑点。结论通过试验表明:依本试验方案所得盐酸小檗碱薄层色谱图,黄柏对照药材溶液、榆黄喷雾剂溶液、盐酸小檗碱对照品溶液斑点边缘清晰,Rf=0.66,重现性好,专属性强,操作简单;本法可为重新制订榆黄喷雾剂质量标准提供参考。
Objective To explore the method of TLC identification of berberine hydrochloride in Yuhuang Spray, and to provide a reference for formulating the quality standard of Yuhuang Spray. Methods According to the TLC test, all samples were spotted on the same silica gel G plate with ethyl acetate - butanone - formic acid - water (10: 6: 1: 1) UV lamp view. Observe the fluorescent speckles in the chromatogram of the test product at the corresponding position of the reference chromatogram. Results for the test, Cortex Phellodendri contrast solution chromatography, the corresponding position in the reference substance chromatography, was the same color fluorescent spots. Conclusion The experiment shows that: according to the TLC chromatogram of berberine hydrochloride solution, the contrast medicine solution of Phellodeouron, elm spray solution and berberine standard solution have sharp edges with spot of Rf = 0.66, good reproducibility, The method has the advantages of strong specificity and simple operation; the present method can provide a reference for redesigning the quality standard of elm spray.