论文部分内容阅读
采用RAPD方法,对40个来自于吉林省代表性稻瘟病病菌的单孢培养物进行生物学特性指纹分析,并与传统生理小种鉴定结果相比较。在筛选的26个引物中,每个引物对代表菌株分别扩增2～12条带。经过0～1聚类分析,在0 6遗传相似水平下,将不同地域和不同生理小种的菌株均划分为8个遗传谱系。病菌通过RAPD方法聚类谱系,未能与其所属生理小种结果形成对应关系,但与对鉴别寄主中的个别品种致病的菌株之间存在一定联系。不同地域的代表菌株经RAPD方法分析结果显示,不同地域的菌株可以组成同一谱系,同一地域内的菌株在系谱图中近缘性更为紧密。
Forty cultivars from a single strain of Pyricularia oryzae in Jilin Province were fingerprinted by RAPD method and compared with the results of traditional races. Among the 26 primers selected, 2 ~ 12 bands were amplified for each of the representative strains. After 0 ~ 1 cluster analysis, the strains of different races and different physiological races were divided into 8 genetic lines at 0 6 genetic similarity level. Pathogenic bacteria clustering lineages by RAPD method failed to form a corresponding relationship with their physiological race results, but there was a relationship between the pathogenic bacteria and individual strains of the host. The results of RAPD analysis of representative strains from different regions showed that the strains from different regions could form the same lineage, and the strains within the same region had closer relationship in the pedigree.