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作者曾经观察到Earle'sMEM培养液中的胎牛血清(FBS)对体外培养的RH株弓形虫入侵MDCK细胞的能力有抑制作用,本次实验进一步探讨FBS的浓度、加入血清的时间及加热处理后对弓形虫入侵细胞的影响。实验每次重复三次并按下列公式计算其
The authors have observed that the Fetal Bovine Serum (FBS) in Earle’s MEM culture medium inhibits the ability of T. gondii to invade MDCK cells cultured in vitro. This experiment further explored the concentration of FBS, the time of adding serum and heat treatment After the invasion of Toxoplasma gondii cells. The experiment is repeated three times each and calculated according to the following formula