传统的人类皮肤致敏预测试验都以豚鼠为模型。豚鼠试验方法很繁复,要求大量动物、笼子和科学的动物管理,而且费用昂贵。近年来一种以测量小鼠耳朵肿胀程度来定量地测定迟发型接触性变态反应的替代试验方法——小鼠耳肿胀试验(MEST)得到了广泛的发展和验证。但文献报道的MEST有很多不同方案,某些易变因素(如小鼠种属、年龄、致敏时间、激发间隔时间等)对试验结果有明显影响。本 Traditional human skin sensitization prediction test guinea pig model. The guinea pig test method is cumbersome, requiring a large number of animals, cages and scientific animal management, but also expensive. In recent years, an alternative test method for quantitatively measuring the delayed contact allergy by measuring the degree of swelling of the mouse ear has been widely developed and validated in the mouse ear edema test (MEST). However, there are many different solutions for MEST reported in the literature. Some of the mutable factors (such as mouse species, age, sensitization time, and excitation interval time) have significant effects on the test results. this