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目的探讨建立并且检测甘草黄酮类有效成分的方法。方法选择甘草粉末提取物,采用高效液相色谱法进行监测和分析。结果该方法能对甘草素样品的甘草素含量进行测定,对甘草素具有良好的分离效果和线性关系。结论高效液相法在检测甘草素含量中操作简单且应用效果良好,可以作为甘草有效成分甘草素的质控标准。
Objective To explore and establish a method for the determination of active ingredients of licorice flavonoids. Methods The licorice powder extract was selected and monitored by high performance liquid chromatography. Results The method was able to determine the content of the glycyrrhizin in the sample of liquiritigenin and has a good separation effect and the linear relationship to the content of the liquiritigenin. Conclusion The method of HPLC for the determination of liquiritigenin has simple operation and good application effect and can be used as the quality control standard of licorice.