It has been proved that there exists a cross coupling between vertical heat turbulent transport and vertical velocity by using linear thermodynamics. This result asserts that the vertical component of heat turbulent transport flux is composed of both the transport of the vertical potential temperature gralient and the coupling transport of the vertical velocity. In this paper, the coupling effect of vertical velocity on vertical heat turbulent transportation is validated by using observed data from the atmospheric boundary layer to determine cross coupling coefficients, and a series of significant properties of turbulent transportation are opened out. These properties indicate that the cross coupling coefficient is a logarithm function of the dimensionless vertical velocity and dimensionless height, and is not only related to the friction velocity u*,but also to the coupling roughness height zwo and the coupling temperature Two of the vertical velocity.In addition, the function relations suggest that only when the vertical velocity magnitude conforms to the limitation |W/u* | ≠ 1, and is above the level zwo, then the vertical velocity leads to the cross coupling effect on the vertical heat turbulent transport flux. The cross coupling theory and experimental results provide a challenge to the traditional turbulent K closure theory and the Monin-Obukhov similarity theory.