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目的观察普罗帕酮对家兔两次缺氧性心脏停搏缺氧时间及心肌超微结构的影响。方法将16只家兔随机分为实验组和对照组。参考气管夹闭窒息法制作心跳骤停模型,夹闭前实验组静脉输注普罗帕酮3mg/kg预处理,对照组静注等剂量生理盐水。记录第1次心脏停搏时间T1。(夹闭气管导管至心脏停搏)和第2次心脏停搏时间T2(第1次心脏停搏复苏成功后15min再次夹闭气管导管至心脏停搏),并在第1次气管导管夹闭后3、6min和复苏后10min时行动脉血气检测。每组各抽取两只家兔分别于第1次夹闭10min以及复苏后第2次夹闭4min时取心脏做心肌超微结构电镜检查。结果实验组第1次缺氧性心脏停搏时间为(744.8±78.1)s,较对照组的停搏时间[(629.6±41.5)s]延长(P<0.05),但两组在复苏时间无显著性差异。实验组第2次缺氧性心脏停搏时间为(439.8±74.7)s,长于对照组的(310.4±26.4)s,P<0.01。实验组心肌细胞内超微结构损害较对照组轻微。结论应用普罗帕酮预处理能够延长在体家兔缺氧性心脏停搏时间,可提高心肌缺氧耐受能力。
Objective To observe the effect of propafenone on hypoxia time and myocardial ultrastructure of two hypoxic cardiac arrest in rabbits. Methods Sixteen rabbits were randomly divided into experimental group and control group. The model of cardiac arrest was made by reference to tracheal asphyxiation. Pre-treatment was performed by intravenous infusion of propafenone 3 mg / kg in the experimental group before occlusion, and normal saline in the control group. Record the first cardiac arrest time T1. (Clipping the endotracheal tube to cardiac arrest) and the second cardiac arrest time T2 (clamping the endotracheal tube to cardiac arrest again 15 minutes after the first cardiac arrest resuscitation), and in the first tracheal tube clamping After 3,6min and 10min after resuscitation arterial blood gas detection. Two rabbits in each group were taken to do myocardial ultrastructure electron microscopy in the first clamping 10min and the second clamping 4min after resuscitation. Results The first hypoxic cardioplegia in the experimental group was (744.8 ± 78.1) s, which was longer than that in the control group [(629.6 ± 41.5) s] (P <0.05) Significant difference. The second hypoxic cardioplegia time in the experimental group was (439.8 ± 74.7) s, which was longer than that in the control group (310.4 ± 26.4) s, P <0.01. Experimental group myocardial cell ultrastructure damage than the control group slightly. Conclusion Pretreatment with propafenone can prolong hypoxaemic cardioplegia time in vivo in rabbits and improve myocardial hypoxia tolerance.