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在单因子试验的基础上,采用了L9(34)正交试验设计探讨了培养基配比、植物生长调节剂的不同浓度、蔗糖量等因素对诱导盐桦(Betula halophila)丛生芽生根的影响。结果表明,对生根率和生根系数的影响大小依次为生长素浓度>培养基配比>蔗糖量;而对于平均根长的影响大小依次为培养基配比>生长素浓度>蔗糖量。筛选出最佳生根培养基为1/2MS,NAA 0.10 mg/L,蔗糖30 g/L。最有利于盐桦无菌苗移栽存活的基质类型为腐殖质土∶珍珠岩=1∶1,成活率达到了64.4%。成活的盐桦组培苗仍具有较好的耐盐性。
On the basis of single factor experiment, orthogonal design of L9 (34) was used to study the effects of medium ratio, different concentrations of plant growth regulators and sucrose content on the rooting of clumps of Betula halophila . The results showed that the effects of rooting rate and rooting coefficient were in order of auxin concentration> medium ratio> sucrose amount, while the effect on average root length was in the order of medium ratio> auxin concentration> sucrose amount. The best medium for rooting was 1 / 2MS, NAA 0.10 mg / L and sucrose 30 g / L. The most favorable substrate for transplanting the sterile seedlings of salt birch was humus soil: perlite = 1: 1, and the survival rate reached 64.4%. Survival salt birch tissue culture seedlings still have good salt tolerance.