Background:The zone of calcified cartilage (ZCC) plays an important role in the pathogenesis of osteoarthritis (OA) but has never been imaged in vivo with magnetic resonance (MR) imaging techniques.We investigated the feasibility of direct imaging of the ZCC in both cadaveric whole knee specimens and in vivo healthy knees using a 3-dimensional ultrashort echo time cones (3D UTE-Cones)sequence on a clinical 3T scanner.Methods:In all,12 cadaveric knee joints and 10 in vivo healthy were collected.At a 3T MR scanner with an 8-channel knee coil,a fat-saturated 3D dual-echo UTE-Cones sequence was used to image the ZCC,following with a short rectangular pulse excitation and 3D spiral sampling with conical view ordering.The regions of interests (ROIs) were delineated by a blinded observer.Singlecomponent T2* and T2 values were calculated from fat-saturated 3D dual-echo UTE-Cones and a Carr-Purcell-Meiboom-Gill (T2 CPMG) data using a semi-automated MATLAB code.Results:The single-exponential fitting curve of ZCC was accurately obtained with R2 of 0.989.For keen joint samples,the ZCC has a short T2* ranging from 0.62 to 2.55ms,with the mean±standard deviation (SD) of 1.49±0.66ms,and with 95％ confidence intervals (CI) of 1.20-1.78 ms.For volunteers,the short T2* ranges from 0.93 to 3.52 ms,with the mean ± SD of 2.09 ± 0.56 ms,and the 95％ CI is 1.43 to 2.74ms in ZCC.Contusions:The high-resolution 3D UTE-Cones sequence might be used to direcdy image ZCC in the human knee joint on a clinical 3T scanner with a scan time of more than 10 min.Using this non-invasive technique,the T2* relaxation time of the ZCC can be further detected.