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作者观察了从感染日本血吸虫的兔粪孵化出来的毛蚴和从阳性钉螺逸出的尾蚴。分别将毛蚴和尾蚴置于3%戊二醛 pH7. 4的磷酸缓冲液中,在4℃下固定2小时。然后用磷酸缓冲液充分洗涤,继以1%四氧化锇在4℃继续固定2小时。经各级浓度的乙醇脱水,通过一系列醋酸盐-乙醇混合液(50%醋酸戊酯)处理,再转入酯酸戊酸。然后将标本置于二氧化碳临界点干燥器中干燥,用碳金镀膜,在 JSM-U3扫描电镜下观察。
The authors looked at the miracidia hatching from rabbit manure infected with Schistosoma japonicum and the cercariae escaping from the positive snail. Metarhizium and cercariae were placed in 3% glutaraldehyde pH7.4 phosphate buffer, fixed at 4 ℃ for 2 hours. It was then washed extensively with phosphate buffer, followed by 1% osmium tetroxide at 4 ° C for 2 hours. After the concentration of ethanol at all levels of dehydration, through a series of acetate - ethanol mixture (50% amyl acetate) treatment, and then into the ester acid valeric acid. The specimens were then placed in a carbon dioxide dry-point desiccator, coated with carbon gold and observed under a JSM-U3 scanning electron microscope.