核酶作为一种特异性抑制转录后基因表达的遗传操作工具 ,已在基因治疗和后基因组研究领域显示出其巨大的应用价值。目前 ,人们正致力于提高核酶在体内的活性 ,以及寻找新的更有价值的核酶结构。本文就在核酶的设计 目标剪切位点的选择 促进核酶剪切活性的额外因子 用体外筛选方法获得新型核酶、在哺乳动物模型中的应用等方面的进展以及核酶研究中应注意的问题作一介绍 As a genetic manipulation tool that specifically inhibits post-transcriptional gene expression, ribozymes have shown great value in gene therapy and post-genomic research. At present, people are working to improve the activity of ribozymes in vivo, as well as to find new and more valuable ribozyme structures. In this paper, we will pay attention to the advances in the design of target ribozyme for ribozyme selection, the extra factors for promoting the ribozyme shearing activity, the in vitro screening method for obtaining novel ribozymes, the application in mammalian models, etc. An introduction to the problem