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炭疽病是鹅掌柴发生最严重的病害之一。本文从四川省成都市采集疑似为炭疽病的鹅掌柴病叶,进行了分离培养,获得培养形态完全不同的2株炭疽菌,致病性测定发现它们都为致病菌。多基因(核糖体内转录间隔区、肌动蛋白、3-磷酸甘油醛脱氢酶、几丁质合成酶、β-微管蛋白、钙调蛋白)的系统发育分析发现,分离获得的2株炭疽菌分别与暹罗炭疽菌(Colletotrichum siamense)和喀斯特炭疽菌(C.karstii)聚在一起,形成一个明显的分支,结合形态学特点,确定引起成都市鹅掌柴炭疽病的病原菌为暹罗炭疽菌和喀斯特炭疽菌,这是关于这两种病原引起鹅掌柴炭疽病的首次报道。本文还研究了不同的回接方式对致病性测定的影响,砂纸擦拭后选取中老叶片用孢子悬浮液接种是对鹅掌柴炭疽病最好的致病性测定方法。
Anthracnose is one of the most serious diseases of Schefflera. In this paper, the leaves of Centella asiatica suspected to be anthrax were collected from Chengdu City, Sichuan Province, and were isolated and cultured to obtain two strains of anthrax with completely different culture patterns. Pathogenicity test showed that all of them were pathogenic bacteria. Phylogenetic analysis of multiple genes (ribosomal transcribed spacer, actin, glyceraldehyde-3-phosphate dehydrogenase, chitin synthase, β-tubulin, calmodulin) found that two isolates of anthrax The bacteria, together with Colletotrichum siamense and C. karstii respectively, formed a distinct branch. Combining the morphological characteristics, the pathogen causing anthracnose in Schefflera of Chengdu was identified as Siam anthrax Bacteria and karst anthracnose, which is the first report of Schefferra foetida on these two pathogens. In this paper, we also studied the impact of different patching methods on the pathogenicity test. After sandpaper was rubbed, the selection of middle and old leaves with spore suspension was the best pathogenicity test for Anthracnose.