论文部分内容阅读
研究螺旋藻中藻胆蛋白、多糖和脂类等药用成分的综合提取新工艺。首先,通过细胞破碎和盐析提取出藻胆蛋白,得率为3.99%,纯度达到0.61。然后用70%的丙酮对藻渣中的脂类进行提取,得率为2.2%。最后提取多糖,应用正交设计得出实验的最佳因素水平为碱溶液浓度为0.1mol/L,提取时间为1h,提取温度为室温。经TCA除蛋白后,用苯酚-硫酸法测定多糖得率达4.02%,多糖纯度为83.2%。
Research Spirulina phycobiliproteins, polysaccharides and lipids and other medicinal ingredients of the new comprehensive extraction process. First, phycobiliproteins were extracted by cell disruption and salting out, with a yield of 3.99% and a purity of 0.61. Then the fat in the algal residue was extracted with 70% acetone, and the yield was 2.2%. Finally, polysaccharides were extracted. The optimal factors for the experiment were obtained by orthogonal design. The concentration of alkaline solution was 0.1mol / L, the extraction time was 1h and the extraction temperature was room temperature. After the protein was removed by TCA, the polysaccharide yield was 4.02% and the polysaccharide purity was 83.2% by phenol-sulfuric acid method.