目的评价2009-2010年上海市甲型H1N1流行性感冒疫苗(甲流疫苗)的免疫学效果。方法以公安人员、中小学生、医务人员这三类重点人群作为免疫学评价的对象,采用分层抽样的方法在上海市4个区县抽取公安人员110人,中小学生146人,医务人员306人,于甲流疫苗接种前和接种后5~6周分别采集外周静脉血,检测甲型H1N1流感(甲流)抗体水平,进行甲流疫苗免疫学效果评价。结果总体上,甲流抗体阳性率由接种前34.9%提高到98.9%,抗体几何平均滴度(GMT)由接种前1∶17提高到1∶351,抗体≥4倍增长率为85.6%。结论 2009年上海市大规模人群接种的甲流疫苗具有良好的免疫学效果。 Objective To evaluate the immunological effects of Influenza A (H1N1) influenza vaccine (H1N1 vaccine) in Shanghai during 2009-2010. Methods Taking the three key groups of public security, primary and secondary school students and medical personnel as the targets of immunological evaluation, stratified sampling method was used to select 110 public security officers, 146 primary and secondary students and 306 medical staff in 4 districts and counties in Shanghai. , Peripheral venous blood was collected before and after 5 ~ 6 weeks after inoculation, and the antibody level of influenza A (H1N1) was detected to evaluate the immunological effects. Results In general, the positive rate of influenza A antibody was increased from 34.9% before inoculation to 98.9%. The geometric mean antibody titer (GMT) increased from 1:17 to 1:351 before inoculation, and the antibody> 4-fold increase rate was 85.6%. Conclusion The 2009 H1N1 flu vaccine vaccinated by large-scale population in Shanghai has a good immunological effect.