A twin-accumulator hydro-pneumatic suspension has been developed based on the off-road vehicle in order to meet the requirements of ride comfort. The working principle and elements construct of the developed suspension are studied. And then,a mathematical model of the developed suspension is built. The influence of twin-accumulator hydro-pneumatic suspension parameters on the vehicle body vertical acceleration,suspension travel and dynamic tyre load is studied by simulation based on a quarter off-road vehicle model. The ride comfort of the vehicle with the developed suspension is studied by a theoretical evaluation; also the ride comfort of the vehicle with twin-accumulator hydro-pneumatic suspension is compared with the one with single accumulator hydro-pneumatic suspension in both time domain and frequency domain. The result shows that the twin-accumulator hydro-pneumatic suspension system gives worthwhile improvements in ride comfort compared with the single accumulator hydro-pneumatic suspension,and it is more suitable for off-road vehicle. A twin-accumulator hydro-pneumatic suspension has been developed based on the off-road vehicle in order to meet the requirements of ride comfort. The working principle and elements construct of the developed suspension are studied. And then, a mathematical model of the developed The influence of twin-accumulator hydro-pneumatic suspension parameters on the vehicle body vertical acceleration, suspension travel and dynamic tire load is studied by simulation based on a quarter off-road vehicle model. The ride comfort of the vehicle with the developed suspension is studied by a theoretical evaluation; also the ride comfort of the vehicle with twin-accumulator hydro-pneumatic suspension is compared with the one with single accumulator hydro-pneumatic suspension in both time domain and frequency domain. -accumulator hydro-pneumatic suspension system gives worthwhile improvements in riding comfort compared with the single accumulator hydro-pneuma tic suspension, and it is more suitable for off-road vehicle.