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[目的]观察大豆异黄酮联合碳酸钙对去势大鼠骨代谢的调节作用。[方法]将60只雌性SD大鼠随机分为6组,分别为假手术组、3个剂量组、碳酸钙组和去卵巢对照组,每组10只。假手术组单纯开腹,其余5组切除双侧卵巢。低、中、高剂量组分别灌胃给予受试物(大豆异黄酮4.8、9.7、29.0 mg/kg BW,碳酸钙25.5、50.9、152.8mg/kg BW),碳酸钙组灌胃给予碳酸钙152.8 mg/kg BW,实验共3个月。每周记录一次体重。实验结束后测定大鼠血清碱性磷酸酶(AKP)和血清钙,取股骨检测股骨中点和远心端骨密度和骨钙含量。[结果]低、中剂量组大鼠股骨远心点骨密度与去卵巢对照组比较显著增加(P﹤0.05),低、高剂量组大鼠股骨骨钙含量与去卵巢对照组比较显著增加(P﹤0.05)。[结论大豆异黄酮联合碳酸钙具有增加去势大鼠骨密度和骨钙含量的作用。
[Objective] To observe the regulatory effect of soybean isoflavone combined with calcium carbonate on bone metabolism in ovariectomized rats. [Methods] Sixty female SD rats were randomly divided into 6 groups: sham operation group, 3 dose groups, calcium carbonate group and ovariectomized control group, 10 in each group. Sham-operated group simply laparotomy, and the remaining 5 groups of bilateral ovaries. The low, medium and high dose groups were given gavage respectively (soy isoflavone 4.8, 9.7, 29.0 mg / kg BW, calcium carbonate 25.5, 50.9, 152.8 mg / kg BW) mg / kg BW, the experiment a total of 3 months. Record the weight weekly. At the end of the experiment, serum AKP and serum calcium were measured. The femur midpoint and distal end BMD and bone calcium content were measured by femur. [Result] Compared with ovariectomized group, the bone mineral density of distal femur of rats in low and middle dose groups increased significantly (P <0.05), and the content of calcium in femur of rats in low and high dose groups increased significantly compared with that in ovariectomized control group P <0.05). [Conclusion Soy isoflavone combined with calcium carbonate can increase bone mineral density and calcium content in ovariectomized rats.