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簇生穗突变体Cl表型为多数枝梗顶端有2或3个小穗簇生在一起.利用扫描电子显微镜观察显示,Cl的功能与水稻枝梗顶部的发育有关,同时Cl影响了顶端小穗的伸长;而Cl突变体中穗粒数有一定降低,提示Cl基因可能对水稻穗粒数也有一定的影响.分别利用Cl与中花11及Cl与浙辐802杂交的F2群体对Cl位点进行遗传定位,Cl位点初步定位在第6染色体的CAPS标记CK0214和SS0324之间.为了进一步精细定位Cl位点,在CK0214和SS0324之间发展了5个CAPS标记,连锁分析表明,Cl与其中2个标记R0674E和C12560紧密连锁,遗传距离分别为0.2和2.1 cM,并且Cl被定位在这两个标记之间.以此为起点,构建覆盖Cl基因区域的PAC重叠群,两个PAC克隆AP004571和AP004236将Cl位点覆盖,物理距离为196 kb,为最终克隆Cl基因奠定了基础.等位性测定显示,Cl与另一个水稻簇生穗突变体Cl2是等位突变.
Cl phenotype of cluster-spike mutant was that 2 or 3 spikelets clustered together at the top of most branch stems.Considered by scanning electron microscopy, the function of Cl was related to the development of the top of rice stem branch, Elongation, while the number of spikelets in Cl mutant decreased to a certain extent, suggesting that Cl may also have some impact on the number of spikelets per panicle. Cl loci were cloned using F2 populations of Zhonghua 11 and Cl and Zhefu 802 respectively And the Cl site was initially mapped between the CAPS markers CK0214 and SS0324 on chromosome 6. To further finely locate the Cl sites, five CAPS markers were developed between CK0214 and SS0324, and the linkage analysis showed that Cl and Two markers, R0674E and C12560, were closely linked with a genetic distance of 0.2 and 2.1 cM, respectively, and Cl was located between the two markers.As a starting point, a PAC contig covering the Cl gene region was constructed and two PAC clones AP004571 And AP004236 cloned the Cl site with a physical distance of 196 kb, which laid the foundation for the cloning of Cl gene.Allege determination showed that Cl was allelic with Cl2 of another rice cluster-spike mutant.