目的建立催化动力学分光光度法测定常用抗肿瘤药顺铂注射液含量的方法。方法在硫酸介质中,进行顺铂催化溴酸钾氧化三溴偶氮氯膦的褪色反应,褪色程度与顺铂含量呈线性关系,利用催化动力学分光光度法进行测定。结果顺铂-三溴偶氮氯膦-溴酸钾反应液的最大吸收峰在530 nm,3~24 ng.mL-1内线性关系良好(r=0.999 1),平均回收率为99.3%,RSD为1.24%(n=3)。结论本方法灵敏度高、操作简便快速,可用于顺铂注射剂的含量测定。 Objective To establish a catalytic kinetic spectrophotometric method for the determination of cisplatin injection. Methods In sulfuric acid medium, cisplatin was used to catalyze the oxidation reaction of bromine tribromide chlorophosphonates with potassium bromate. The degree of discoloration was linear with the content of cisplatin, and was determined by catalytic kinetic spectrophotometry. Results The maximum absorption peak of cisplatin-TBBA-KBr solution had a good linearity (r = 0.999 1) at 530 nm and 3 ~ 24 ng · mL-1 with an average recovery of 99.3% 1.24% (n = 3). Conclusion The method is sensitive, simple and rapid, and can be used to determine the content of cisplatin injection.