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目的：分析我国山丘和平原疫区利什曼原虫分离株基因组ＤＮＡ（ｎＤＮＡ）的多态性。方法：对基因组ＤＮＡ进行内切酶酶切、Ｓｏｕｔｈｅｒｎ杂交、染色体定位。探针采用地高辛标记。结果：采用ｇｐ６３探针，显示杜氏利什曼原虫（Ｌｅｉｓｈｍａｎｉａｄｏｎｏｖａｎｉ，Ｌ．ｄ．）江苏人株和Ｌ．ｄ．ＪｅｄｄａｈｎＤＮＡ之间有相似的染色体杂交带，Ｌ．ｄ．四川人株和Ｌ．ｉｎｆａｎｔｕｍｎＤＮＡ之间有相似的染色体杂交带；采用β－ｔｕｂｕｌｉｎ探针，显示Ｌ．ｄ．江苏人株与Ｌ．ｄ．ＪｅｄｄａｈｎＤＮＡ之间有两条相似的染色体杂交带，Ｌ．ｄ．四川犬株与Ｌ．ｄ．甘肃犬株ｎＤＮＡ之间有三条相似的染色体杂交带、与Ｌ．ｄ．汶川人株ｎＤＮＡ之间有两条相似的染色体杂交带。结论：提示平原疫区的江苏人株与Ｌ．ｄ．Ｊｅｄｄａｈ之间存在同源性，山丘疫区的Ｌ．ｄ．四川人株与Ｌ．ｉｎｆａｎｔｕｍ之间存在同源性，Ｌ．ｄ．四川犬株与Ｌ．ｄ．甘肃犬株之间存在同源性、与Ｌ．ｄ．汶川人株之间既存在同源性又存在差异，山丘疫区与平原疫区分离株之间存在异源性
Objective: To analyze the genomic DNA (nDNA) polymorphism of Leishmania isolates in hills and plains of China. Methods: Genomic DNA was digested with restriction endonuclease, Southern blot and chromosomal localization. Probe using digoxin labeling. Results: Leishmaniadonovani, L.d. Jiangsu strain and L strain were displayed using the gp63 probe. d. JeddahnDNA similar chromosomes between the hybridization, L. d. Sichuan strain and L. Similar chromosomal hybridization bands were found between infantumnDNA; beta-tubulin probes were used to show L. d. Jiangsu strain and L. d. There are two similar hybridized bands between JeddahnDNA. d. Sichuan dog strains and L. d. Gansu canine nDNA between the three similar chromosomes, and L. d. There are two similar chromosome hybridization bands between Wenchuan human strains nDNA. Conclusion: It is suggested that Jiangsu strain and L. in plague-affected area. d. There is homology between Jeddah and L. in the affected area. d. Sichuan strains and L. There is homology between infantum, L. d. Sichuan dog strains and L. d. Gansu dog strains exist between homology, and L. d. There is both homology and difference between the Wenchuan strains, and there is a heterogeneity between the epidemic strains in the hills and the plague-endemic regions