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目的研究高尔基体蛋白73(Golgi protein 73,GP73)、甲胎蛋白(alpha-fetoprotein,AFP)单独及联合使用在原发性肝癌中的诊断意义.方法选择我院2011年4月-2012年10月门诊、住院患者和健康体检人群,分别用酶联免疫吸附法和化学发光免疫分析法检测47例原发性肝癌、45例肝硬化、387例乙肝携带者、528例健康对照组血清GP73与AFP水平,分析两指标各组间差别,绘制其诊断原发性肝癌的ROC曲线.结果原发性肝癌、肝硬化、乙肝携带者、健康对照组GP73分别为174.22±119.22、127.98±39.71、74.51±40.13、47.08±22.75ng/ml,各组间均存在显著差异.原发性肝癌、肝硬化、乙肝携带者、健康对照组AFP中位数分别为27、3.2、2.8、2.6ng/ml,原发性肝癌组高于其他各组,其他各组间无统计学差异.GP73诊断原发性肝癌ROC曲线的曲线下面积、敏感度、特异度、Youden指数分为别0.819、0.574、0.966、0.540;AFP分别为0.825、0.745、0.846、0.591.两者平行联用敏感度、特异度、Youden指数分别为0.851、0.815、0.666;序贯联用以上指数分别为0.362、0.990、0.352.结论 GP73、AFP两者平行联用敏感度0.851,特异度0.815,对诊断HCC有较好的诊断性能.“,”Objective To assess diagnostic value of Golgi protein 73(GP73) and alpha-fetoprotein(AFP) in hepatic cellular carcinoma(HCC). Methods Among wards, out-patients and healthy check people in our hospital from April 2011 to October 2012, 47 HCC, 45 hepatic cirrhosis, 387 HBV carriers and 528 healthy control people were involved in the study. GP73 in serum was measured by ELISA and AFP was measured by ADVIA Centaur XP Immunoassay System. The sensitivity, specification were calculated. Results GP73 in HCC, hepatic cirrhosis, HBV carriers and healthy control people were 174.22 ±119.22, 127.98±39.71, 74.51±40.13, 47.08±22.75ng/ml and showed significance difference. The medians of AFP in HCC, hepatic cirrhosis, HBV carriers and healthy control people were 27, 3.2, 2.8 and 2.6ng/ml. AFP in HCC showed significance difference. The summary estimates for serum GP73 in HCC in the study included were as follows: area under curve ROC, 0.819; sensitivity, 0.574; specificity, 0.966 and Youden index 0.540. Those of AFP were 0.825, 0.745, 0.846 and 0.591. Sensitivity, specificity and Youden index for parallel combination of GP73 and AFP in HCC are 0.851, 0.815 and 0.666; those for sequential combination are 0.362, 0.990 and 0.352. Conclusion With sensitivity 0.851 and specificity 0.815 for parallel combination of GP73 and AFP, it has a fine diagnostics performance in HCC.