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该文探讨甲基化Cp G结合域蛋白2(methyl-Cp G-binding domain protein 2,MBD2)对人胰腺癌细胞增殖和迁移的影响。将干扰质粒MBD2 sh RNA转染入MBD2高表达的Pa Tu8988细胞和SW1990细胞中,运用CCK-8法检测细胞的增殖活性并绘制生长曲线,克隆形成实验检测细胞克隆形成率,Transwell检测细胞迁移率。结果显示,下调MBD2后,Pa Tu8988细胞中MBD2基因的表达量明显降低,细胞增殖减慢,克隆形成率降低,细胞迁移率减少;在SW1990细胞中下调MBD2基因后,得到类似结果。由此说明,沉默MBD2基因可以抑制胰腺癌细胞Pa Tu8988和SW1990的增殖和迁移,为进一步研究MBD2在胰腺癌细胞增殖与迁移中的作用机制奠定了基础。
This article investigates the effect of methyl-CpG-binding domain protein 2 (MBD2) on the proliferation and migration of human pancreatic cancer cells. The interference plasmid MBD2 sh RNA was transfected into Pa Tu8988 cells and SW1990 cells with high expression of MBD2. The proliferation activity of the cells was detected by CCK-8 assay and the growth curve was drawn. Clone formation assay was used to detect the cell clone formation rate. . The results showed that the expression of MBD2 gene was significantly decreased in Pa Tu8988 cells after down-regulation of MBD2, the cell proliferation was slowed down, the colony formation rate was reduced, and the cell migration rate was reduced. Similar results were obtained after MBD2 gene was down-regulated in SW1990 cells. Thus, silencing MBD2 gene can inhibit the proliferation and migration of pancreatic cancer cells Pa Tu8988 and SW1990, which laid the foundation for further study on the mechanism of MBD2 in the proliferation and migration of pancreatic cancer cells.