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利用薄层扫描法与薄层层离-分光光度法分析古方四妙勇安汤中阿魏酸的含量,采用硅胶G-0.8%-CMC-Na薄层板,以苯-乙酸乙酯-甲醇(16:4:1)外加3滴甲酸为展开剂,分别用岛津CS-9000型薄层扫描仪和752型紫外分光光度计测定四妙勇安汤中阿魏酸的含量,两种方法所测得的结果比较接近。
Thin-Layer Scanning Method and TLC-Spectrophotometric Analysis of Ferulic Acid Content in Gufang Simiao Yongan Decoction Using Silica Gel G-0.8%-CMC-Na TLC Plate with Benzene-Ethyl Acetate -Methanol (16:4:1) plus 3 drops of formic acid as developing solvent. The content of ferulic acid in Simoo Yongan Decoction was determined using Shimadzu CS-9000 thin-layer scanner and 752 UV spectrophotometer. The results obtained by these methods are relatively close.