乙醇发酵──完全细胞截留渗透汽化膜分离耦合过程

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设计构建了发酵罐有效容积为250mL,与一有效膜面积为50cm2的平板渗透汽化池耦合的膜生物反应系统,实验考察了该系统中的完全细胞截留连续乙醇发酵过程.并与未耦合之间歇发酵过程相比较:即使该系统以低稀释速率D=0.05h-1操作,产物乙醇浓度和体积产率亦可提高约两倍,且底物葡萄糖消耗接近100%.流加葡萄糖浓度越高,体积产率改善越大,但乙醇得率系数有所下降.在流加糖浓度为158和289.4g/L时,乙醇体积产率改善,分别为1.58倍和1.86倍,乙醇得率系数分别为0.45和0.395.耦合过程中进行乙醇发酵时实测的渗透汽化性能较乙醇-水所测性能要差.随着发酵过程的进行,渗透通量下降显著,而分离系数基本稳定,这可能与酵母细胞、无机盐及不挥发性副产物的累积所产生的膜污染有关. A membrane bioreactor system was designed and constructed with an effective volume of 250mL and a plate pervaporation tank with an effective membrane area of ​​50cm2. The complete cell-cut continuous ethanol fermentation process in the system was investigated experimentally. And compared to an uncoupled batch fermentation process: The product ethanol concentration and volumetric yield can be increased by about a factor of two and the substrate glucose consumption nearly 100%, even though the system is operated at a low dilution rate of D = 0.05h-1. The higher the concentration of glucose and glucose, the greater the improvement of volumetric yield, but the decrease of ethanol yield coefficient. At the concentrations of 158 and 289.4 g / L, the ethanol yield was improved by 1.58 and 1.86 times, respectively. The yield coefficients of ethanol were 0.45 and 0.395, respectively. The measured pervaporation performance under ethanol fermentation during coupling is poorer than that measured with ethanol-water. With the fermentation process, the permeate flux decreased significantly, while the separation factor was basically stable, which may be related to the membrane fouling caused by the accumulation of yeast cells, inorganic salts and non-volatile by-products.
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