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丝氨酸/苏氨酸蛋白磷酸酶6亚基(catalytic subunits of Ser/Thr protein phosphatase 6,PP6C)是PP6全酶的催化亚基。在模式植物拟南芥中的研究表明,PP6C参与生长素极性运输、脱落酸信号转导和光信号转导途径介导的开花调控。为了明确玉米丝氨酸/苏氨酸蛋白磷酸酶6亚基(ZmPP6C)的蛋白结构特征与同源蛋白间的进化关系,采用RT-PCR方法克隆了ZmPP6C的全长基因。序列分析表明,ZmPP6C开放阅读框为912个核苷酸,编码303个氨基酸残基,包含PP2A的催化亚基PP2Ac结构域;系统进化树分析表明,PP6C蛋白在进化上较为保守,并且与高粱的PP6C蛋白相似性更高。对玉米自交系B73的ZmPP6C基因进行器官特异性表达分析表明,其表达量在成株期叶片中最高,是根中的7.9倍;ZmPP6C能够响应不同光质处理,且受远红光和红光的影响较大;也能响应长日和短日处理,在长日条件下的光照和黑暗阶段各有一个明显的表达高峰,在短日条件下的光照和黑暗阶段分别有2个和3个表达峰值;同时,ZmPP6C还响应高渗透、盐渍和淹水等胁迫处理,出现明显的上调表达。结果表明,ZmPP6C在玉米光信号转导、开花诱导与胁迫应答中发挥重要作用,其分子与生化机制值得进一步探讨。
The catalytic subunits of Ser / Thr protein phosphatase 6 (PP6C) is the catalytic subunit of PP6 holoenzyme. Studies in the model plant Arabidopsis indicate that PP6C is involved in flowering regulation mediated by polar auxin transport, abscisic acid signaling and optical signal transduction pathways. In order to clarify the relationship between the protein structural characteristics of ZmPP6C and the homologous proteins, the full-length ZmPP6C gene was cloned by RT-PCR. Sequence analysis showed that the open reading frame of ZmPP6C was 912 nucleotides and encoded 303 amino acid residues, including PP2Ac, a catalytic subunit of PP2A. Phylogenetic tree analysis showed that PP6C protein was more evolutionarily conserved, PP6C protein is more similar. The organ-specific expression analysis of the ZmPP6C gene in maize inbred line B73 showed that the expression level of ZmPP6C gene was the highest in adult leaves, which was 7.9 times that of the roots. ZmPP6C was able to respond to different light treatments and was affected by far red light and red But also respond to the long-day and short-day processing, there is a clear peak of expression in the light and dark phases under long-day conditions. There are 2 and 3 respectively in the short-day light and dark phases The expression of ZmPP6C was also up-regulated in response to high stress, salinity and flooding treatments. The results showed that ZmPP6C played an important role in light signal transduction, flowering induction and stress response in maize, and its molecular and biochemical mechanisms deserved to be further explored.