论文部分内容阅读
目的:建立高效液相色谱法测定复方清带灌注液中苦参碱、槐定碱、蛇床子素的含量。方法:采用HPLC法,苦参碱、槐定碱:色谱柱为氨基键合硅胶(150 mm×4.6 mm,5μm);以乙腈-甲醇-3%磷酸溶液(86∶5∶9)为流动相;柱温:30℃;流速:1.0 ml·min-1;检测波长:210 nm。蛇床子素:色谱柱为十八烷基硅烷键合硅胶(150 mm×4.6 mm,5μm),以乙腈-水(65∶35)为流动相,柱温:30℃;流速:1 ml·min-1;检测波长:322 nm。结果:苦参碱在0.270~2.027μg线性关系良好(r=0.999 9),平均加样回收率101.83%(RSD=1.92%,n=6);槐定碱在0.041~0.306μg线性关系良好(r=0.999 9),平均加样回收率97.98%(RSD=1.75%,n=6);蛇床子素在0.020~0.136μg线性关系良好(r=0.999 9),平均加样回收率102.77%(RSD=1.09%,n=6)。结论:方法简便,准确,重复性好,可用于复方清带灌注液苦参碱、槐定碱、蛇床子素的含量测定。
OBJECTIVE: To establish a method for the determination of matrine, sophoridine and osthole in compound Qinghai perfusion fluid by HPLC. Methods: The HPLC method was used. Matrine and sophoridine were immobilized on the column as amino-bonded silica gel (150 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-methanol-3% phosphoric acid solution (86:5:9) ; Column temperature: 30 ℃; flow rate: 1.0 ml · min-1; detection wavelength: 210 nm. Osthol: octadecylsilane bonded silica gel (150 mm × 4.6 mm, 5 μm) with acetonitrile-water (65:35) as mobile phase at a column temperature of 30 ℃ and a flow rate of 1 ml · min -1; detection wavelength: 322 nm. Results: The linear relationship of matrine between 0.270 and 2.027μg was good (r = 0.999 9), the average recovery was 101.83% (RSD = 1.92%, n = 6), sophoridine was 0.041-0.306μg The average recovery was 97.98% (RSD = 1.75%, n = 6). The osthole showed a good linearity (r = 0.999 9) at 0.020-0.136 μg, with an average recovery of 102.77% (r = 0.999 9) RSD = 1.09%, n = 6). Conclusion: The method is simple, accurate and reproducible. It can be used for the determination of matrine, sophoridine and osthole in the compound Qinghai perfusion fluid.