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探讨肝纤维化发生过程中，细胞骨架蛋白—结蛋白（Ｄｍ）和α－平滑肌肌动蛋白（α－ＳＭＡ）阳性细胞动态变化的规律以及这些阳性细胞之间的关系。方法用四氯化碳辅以低胆碱饲料诱导大鼠肝纤维化，应用免疫组化、电镜技术和图像分析方法对肝纤维化过程中出现的Ｄｍ和α－ＳＭＡ阳性细胞进行定位和定量研究。结果α－ＳＭＡ阳性细胞的变化几乎与Ｄｍ阳性细胞同步，两者于实验早期均有增生，但前者少于后者，至纤维间隔大量形成时（１２周），两者增生均达到最高峰，但前者多于后者，以后两者均逐步下降；从形态分析，Ｄｍ和α－ＳＭＡ阳性细胞主要为贮脂细胞（ＦＳＣ），部分卵圆细胞和新生胆管上皮细胞也表达此两种骨架蛋白。结论除了ＦＳＣ以外，α－ＳＭＡ和Ｄｍ阳性卵圆细胞也可能在肝纤维化发生发展中起重要作用。
To investigate the dynamic changes of cytoskeletal protein-desmin (Dm) and α-smooth muscle actin (α-SMA) positive cells in the process of hepatic fibrosis and the relationship between these positive cells. Methods CCl4 supplemented with low choline feed to induce hepatic fibrosis in rats. Immunohistochemistry, electron microscopy and image analysis were used to locate and quantify Dm and α-SMA positive cells in liver fibrosis . Results The changes of α-SMA positive cells were almost synchronous with that of Dm positive cells. Both of them showed hyperplasia in the early stage of the experiment, but the former was less than the latter. When the fibrin septa were formed in large quantities (12 weeks) However, the former was more than the latter, and the latter both decreased gradually. From the morphological analysis, Dm and α-SMA positive cells were mainly fat storage cells (FSC), and some oval cells and nascent biliary epithelial cells also expressed these two scaffold proteins . Conclusion In addition to FSC, α-SMA and Dm-positive oval cells may also play an important role in the development of liver fibrosis.