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目的探讨骨灵膏及其拆方制剂对通过冷-固联合诱导法制作的骨关节炎(OA)大鼠模型作用的机制研究。方法 60只雌性SD大鼠随机分为骨灵膏组、骨膏组、灵膏组、塞来昔布组、模型组及正常组六组,每组10只,除正常组外,其余各组采用冷固法6周建立骨关节炎模型。造模成功后,除正常组和模型组分别给予生理盐水灌胃外,其余组分别给予骨灵膏、骨膏、灵膏及塞来昔布灌胃,给药10周后,取大鼠血清、膝关节软骨组织及滑膜。镜下观察关节软骨病理变化;X片检测关节影像学改变;ELISA检测血清TNF-a、IL-1β、透明质酸(HA)、Ig M含量;TUNEL法检测关节软骨及滑膜细胞凋亡情况。结果冷固法制作OA模型X影像学、关节肿胀情况、软骨组织病理损伤及细胞凋亡和生化代谢改变均符合OA的特征,表明造模成功;骨灵膏可以减轻OA软骨组织浅表至钙化层各层的病理损伤和下调软骨及滑膜细胞凋亡,其中骨灵膏与骨膏、灵膏比较,软骨及滑膜细胞凋亡明显下降(P<0.01),骨灵膏及其拆方制剂骨膏组、灵膏组TNF-a、IL-1β的含量较模型组显著降低(P<0.01);骨灵膏组HA的含量较骨膏、灵膏及塞来昔布组明显增加(P<0.01);各组Ig M的含量无明显差异。结论冷-固联合诱导法能制作理想的OA大鼠模型,骨灵膏可能通过减轻机体炎症损伤、降低关节软骨HA的耗损、抑制关节滑膜及软骨细胞凋亡等多个途径阻止了OA关节软骨退行性变导致的关节损伤,骨灵膏的上述作用优于其拆方制剂骨膏、灵膏及塞来昔布。
OBJECTIVE To study the mechanism of the effect of Gu Ling Pian and its disassembled preparations on osteoarthritis (OA) rat model induced by cold-solid induction. Methods Sixty female Sprague-Dawley rats were randomly divided into six groups: Gu Ling Gum group, Gugu cream group, Ling cream group, Celecoxib group, model group and normal group. Ten rabbits in each group were divided into normal control group, Osteoarthritis model was established by cold-fixation for 6 weeks. After the success of modeling, the rats in the normal group and the model group were given gavage respectively, and the other groups were given Gualutsu, bone plaster, Lingzhi and celecoxib, respectively. After 10 weeks of administration, the serum of rats , Knee joint cartilage and synovium. The pathological changes of articular cartilage were observed under microscope. The changes of articular cartilage were observed by X-ray. The contents of TNF-a, IL-1β, HA and IgM were detected by ELISA. The apoptosis of articular cartilage and synovial cells were detected by TUNEL. . Results OA method X-ray imaging, swollen joints, pathological damage of cartilage tissue and changes of apoptosis and biochemical metabolism were all consistent with the characteristics of OA in cold-solid method, which showed that the modeling was successful. Gingivall could reduce the superficial to calcified OA cartilage Layer of layers of pathological damage and down-regulation of cartilage and synovial cell apoptosis, in which the Bone Gingse and bone plaster, Lingzhi comparison, cartilage and synovial cell apoptosis decreased significantly (P <0.01), Guling and its demolition The content of TNF-a and IL-1β in the preparations of Gugu group and Lingzhi group was significantly lower than that in the model group (P <0.01); the content of HA in Guluoling group was significantly higher than that in Guguogan, Lingzhi and Celecoxib group P <0.01). The content of Ig M in each group had no significant difference. CONCLUSION: Cold-solid induction can produce ideal OA model in rats. Osteoporosis can prevent the OA joint by ameliorating the inflammatory injury, reducing the HA of the articular cartilage, inhibiting the synovial and chondrocyte apoptosis, Cartilage degeneration caused joint damage, the above-mentioned role of Gualutao is superior to its disassemble preparations of bone plaster, cream and celecoxib.