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目的探讨人特异的胰岛素放射免疫分析试剂盒(Ins-RIA)检测小型猪血清胰岛素水平的可行性。方法采集219份巴马小型猪血清标本,分别采用人Ins-RIA和猪特异的胰岛素ELISA试剂盒(Ins-ELISA)测定血清胰岛素水平。分析两种方法检测猪胰岛素水平的相关性,随机抽取80%(175份)的数据进行直线回归分析,以Ins-ELISA为因变量,Ins-RIA为自变量,建立回归方程,再用剩余20%(44份)的数据对回归方程进行回代验证。结果在巴马小型猪,猪Ins-ELISA检测值低于人Ins-RIA检测值,分别为(15.32±15.50)μIU/mL及(32.31±21.74)μIU/mL,差异有统计学意义(P<0.000 1);预测猪Ins-ELISA检测胰岛素的回归方程为InsELISA(μIU/mL)=-7.29+0.70×Ins-RIA(μIU/mL)(R~2=0.94);在回代验证中,Ins-ELISA的预测值和实际检测值之间的平均差异为17.18%,预测值与实际测量值之间的吻合程度较好(P>0.05)。结论巴马小型猪ELISA法检测的血清胰岛素水平可以用人胰岛素RIA法检测的血清胰岛素水平来进行估计。
Objective To investigate the feasibility of using Ins-RIA to detect serum insulin in miniature pigs. Methods Seventy-two Bama miniature pig serum samples were collected for determination of serum insulin levels by using human Ins-RIA and pig-specific insulin ELISA kit (Ins-ELISA). Analysis of the correlation between the two methods to detect the level of insulin in pigs, the data of 80% (175) were randomly selected for linear regression analysis. The regression equation was established with Ins-ELISA as the dependent variable and Ins-RIA as the independent variable, and the remaining 20 % (44 copies) of the data on the regression equation validation. Results The detection value of Ins-ELISA in Bama miniature pigs was lower than that of human Ins-RIA (15.32 ± 15.50) μIU / mL and (32.31 ± 21.74) μIU / mL, respectively, with statistical significance (P < 0.000 1). The regression equation for predicting Insulin in pig Ins-ELISA was InsELISA (μIU / mL) = 7.29 + 0.70 × Ins-RIA (μIU / mL) The average difference between -ELISA predicted value and the actual detection value was 17.18%, and the predicted value was in good agreement with the actual measurement value (P> 0.05). Conclusion The level of serum insulin detected by ELISA in Bama miniature pigs can be estimated by the level of serum insulin measured by human insulin RIA method.