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目的预测肿瘤抗原MAGE-A亚家族的B细胞表位,为基于多靶点的肿瘤疫苗设计提供依据。方法基于MAGE亚家族各成员蛋白质序列,采用Kyte-Doolittle的亲水性方案,Emini方案,Karplus方案和Jameson-wolf抗原指数方案,并辅以MAGE蛋白的二级结构柔性区域分析,预测MAGE基因家族的B细胞共同表位。结果共预测出了5条共同表位,且部分B细胞表位高度相似或一致。结论二级结构与B细胞表位相结合的预测方法为一种高效、准确的表位预测方法,可为肿瘤治疗性疫苗的设计提供实验依据。
Objective To predict the B cell epitope of the MAGE-A subfamily of tumor antigens and provide evidence for the design of multi-target tumor vaccines. Methods Based on the protein sequence of each member of MAGE subfamily, the Kyte-Doolittle hydrophilicity scheme, Emini scheme, Karplus scheme and Jameson-wolf antigen index scheme were combined with the secondary structure flexible region analysis of MAGE protein to predict the MAGE gene family B cell common epitope. Results A total of five common epitopes were predicted, and some of the B cell epitopes were highly similar or consistent. Conclusion The predictive method for the combination of secondary structure and B cell epitopes is an efficient and accurate method for predicting epitopes, which can provide experimental evidence for the design of therapeutic vaccines for tumors.