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将健叶和黄龙病叶叶柄用保险刀片徒手切片,然后在透射式荧光显微镜下检查,便可见到健叶叶柄的木质部导管细胞壁发出黄色的荧光,韧皮纤维细胞则发出绿色荧光,而病叶叶柄韧皮部中有一至多个鲜明的黄色或黄绿色荧光团块。而此种特异性的荧光,在健叶柄韧皮部中是没有的,其它由病毒、类病毒、细菌、真菌等病原引起的病叶柄韧皮部中亦未发现有这种特异性荧光。值得注意的,在取样时必须是完全老熟的黄化叶片,而
Will Kin leaf and yellow dragon leaf petiole with insurance blade freehand slice, and then under the transmission fluorescence microscope examination, we can see the healthy leaf petiole xylem catheter cell wall yellow fluorescence, bovine fibroblasts are emitting green fluorescence, and diseased leaves Petiole phloem one or more distinct yellow or yellow-green fluorophores. However, this specific fluorescence was absent in the phloem of healthy petiole and no other specific fluorescence was found in other phloem of petioles caused by viruses, viruses, bacteria, fungi and other pathogens. It is noteworthy that in the sampling must be completely mature etiolated leaves, and