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利用农杆菌介导法,将菊芋凝集素基因(Helanthus tuberosusagglutinin,HTA)转入常规棉花,获得转抗蚜虫基因棉花再生株。分子检测表明,外源基因已整合在转化棉花受体材料中,转菊芋凝集素基因棉花对棉蚜具有一定的抗性。
Using Agrobacterium-mediated method, the gene of HeLaus tuberosusagglutinin (HTA) was transferred into cotton and the cotton aphid-resistant gene cotton was regenerated. Molecular tests showed that the exogenous genes have been integrated into the transformed cotton receptor material, and the transgenic cotton with the Jerusalem artichoke agglutinin gene has certain resistance to cotton aphid.