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目的 应用感染曼氏血吸虫 (利比里亚株 )的小鼠观察蒿甲醚单剂量与效应的关系 ,虫体肝移及蒿甲醚所引起的虫的形态学和组织病理学变化。 方法 感染 2 1d童虫的小鼠一次口服蒿甲醚 12 .5mg/kg至 60 0mg/kg不同剂量 ,治后 2 8d剖检观察各组虫数。感染 46d或 70d成虫的小鼠一次口服蒿甲醚 40 0mg/kg后 8～ 14d ,观察虫体肝移及其形态和组织病理学变化。 结果 蒿甲醚对 2 1d童虫的最低有效剂量为 2 0 0mg/kg ,减虫率为 81%。用蒿甲醚治疗后 8h成虫开始肝移 ,3～ 7d全部肝移 ,14d有 3 1%的虫返回肠系膜静脉。成虫虫体萎缩 ,咽部扩大 ,肠管膨胀及其色素减少。雌虫局部体表受损 ,白细胞附着 ,卵巢及卵黄腺变性退化 ,以及雄虫睾丸萎缩等。在肝内的虫体被嗜酸粒细胞为主的炎细胞包围和浸润。 结论 蒿甲醚对小鼠曼氏血吸虫 2 1d童虫的最低有效剂量为 2 0 0mg/kg ,可引起曼氏血吸虫成虫萎缩、退化或死亡。在肝内受损的虫体主要是被嗜酸粒细胞包围和侵袭所致。
OBJECTIVE: To investigate the relationship between single dose and effect of artemether and the morphological and histopathological changes of worms caused by liver transplantation and artemether in mice infected with Schistosoma mansoni (Liberia strain). Methods Mice infected with 2 1 d schistosomiasis were treated with different doses of artemether 12.5 mg / kg to 60 mg / kg orally, and the number of each group was observed by necropsy 28 days after the operation. Mice infected with adult 46d or 70d once oral administration of artemether 40 0mg / kg 8 ~ 14d, to observe the liver movement and morphology and histopathological changes. Results The lowest effective dose of artemether for 21 days was 200 mg / kg and worm reduction rate was 81%. After 8 hours of treatment with artemether, the adults started to undergo liver transplantation, all of them were transplanted from 3 to 7 days, and 41% of the worms returned to the mesenteric vein on day 14. Adult worm body atrophy, enlargement of the pharynx, intestinal dilation and pigment reduction. Local damage to the female body surface, leukocyte attachment, degeneration of ovarian and yolk glands, as well as male testicular atrophy. The parasites in the liver are surrounded and infiltrated by eosinophils. Conclusions The lowest effective dose of artemether against Schistosoma mansoni 21d was 20 000 mg / kg, which caused the atrophy, regression or death of adult Schistosoma mansoni. The parasites that are damaged in the liver are mainly surrounded and infiltrated by eosinophils.