目的 :建立特异性的检测严重急性呼吸综合征 (SARS)相关抗体的血清学方法。 方法 :制备 SARS患者肺组织包被抗原 ,采用酶联免疫吸附试验 (EL ISA)检测 SARS患者血清中特异性抗体。 结果 :SARS患者血清中存在 SARS相关的抗人肺组织抗体 ,其阳性率达 92 .86 %(2 6 / 2 8) ,正常献血员为 11.4 3%(12 / 10 5 ) ,统计学分析表明 ,SARS相关抗体的检出率在患者和献血员之间存在明显差异。 结论 :SARS患者血清中存在抗肺组织抗体 ,该抗体的存在提示机体免疫反应可能参与了疾病病理损伤过程 ;检测该抗体可能有助于 SARS的早期诊断 Objective: To establish a specific serological method for the detection of antibodies related to severe acute respiratory syndrome (SARS). Methods: The antigens of lung tissue were prepared from patients with SARS. The serum anti-SARS antibodies were detected by enzyme-linked immunosorbent assay (ELISA). Results: SARS-associated anti-human lung antibodies were found in sera of patients with SARS, the positive rate was 92.86% (26/28) and the normal blood donors was 11.4 3% (12/105). The statistical analysis showed The prevalence of SARS-associated antibodies was significantly different between patients and donors. CONCLUSIONS: Anti-lung antibodies exist in the serum of patients with SARS. The presence of this antibody suggests that the immune response may be involved in the pathogenesis of SARS. Detecting this antibody may contribute to the early diagnosis of SARS