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目的探讨转化生长因子β(TGFβ)超家族的细胞内信号转导分子Smad2、Smad4蛋白在腺嘌呤所致雄性不育的大鼠睾丸内定位、分布及作用机制。方法应用免疫组织化学SABC法检测正常成年及腺嘌呤(300mg kg)灌胃后第7、14、21d大鼠睾丸中Smad2、Smad4的定位和分布,并通过图像分析系统进行统计学分析,同时应用放射免疫法测定血清中睾酮(T)、促黄体激素(LH)和促卵泡激素(FSH)的含量。结果Smad2免疫阳性产物位于实验大鼠各级生精细胞及支持细胞的胞质内,Samd4则位于间质细胞的胞质内;灌胃后第21d大鼠睾丸中Smad2、Smad4表达明显高于正常成年及7、14d大鼠,同时,血清中T较正常成年及7、14d大鼠明显降低,LH、FSH明显增高。结论腺嘌呤所致雄性不育可能与Smad2在大鼠睾丸生精细胞中表达增强及Smad4在睾丸间质细胞中表达增强,直接或间接影响睾丸的生精功能有关。
Objective To investigate the localization, distribution and mechanism of intracellular signal transduction molecules Smad2 and Smad4 in transforming growth factor β (TGFβ) superfamily in testis of adenine induced male rats. Methods Immunohistochemical SABC method was used to detect the localization and distribution of Smad2 and Smad4 in testis of rats on the 7th, 14th, 21st and 21st days after normal adult and adenine (300mg / kg) administration. The data were analyzed by image analysis system and applied simultaneously The content of testosterone (T), luteinizing hormone (LH) and follicle stimulating hormone (FSH) in serum were determined by radioimmunoassay. Results The Smad2 immunopositive product was located in the cytoplasm of spermatogenic cells and supporting cells at all levels in experimental rats, while the expression of Smad4 was located in the cytoplasm of interstitial cells. The expression of Smad2 and Smad4 in testis of rats on day 21 was significantly higher than that of normal Adult and 7,14 d rats, meanwhile, serum T was significantly lower than normal adult and 7 and 14 d rats, LH and FSH were significantly increased. Conclusion Adenine-induced male sterility may be related to the increased expression of Smad2 in rat spermatogenic cells and the increased expression of Smad4 in testicular stromal cells, which directly or indirectly affect the testicular spermatogenesis.