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目的:通过正交试验法优化消肿止痛中药复方的醇提工艺。方法:建立HPLC法,以乙醇浓度、乙醇用量、回流时间、回流次数为考查因素,提取液中大黄素含量为评价指标,进行正交试验,采用SPSS统计软件分析实验结果。结果:得到最佳提取工艺为10倍量90%乙醇提取120 min,提取3次。结论:通过正交试验法优化消肿止痛中药复方的醇提工艺后可得到更佳的提取效果,进而提高复方的临床疗效。
OBJECTIVE: To optimize the alcohol extraction process of traditional Chinese medicine for swelling and relieving pain by orthogonal test. Methods: The HPLC method was established. The emodin content in the extract was taken as the evaluation index by the ethanol concentration, the amount of ethanol, the reflux time and the reflux times. The orthogonal test was carried out. The experimental results were analyzed by SPSS statistical software. Results: The best extraction process was 10 times the amount of 90% ethanol extracted 120 min, extracted three times. Conclusion: Orthogonal test method can be used to optimize the alcohol extraction process of traditional Chinese medicine for swelling, relieving pain, and improve the clinical curative effect of compound prescription.