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利用甲基化敏感扩增多态性(Methylation-sensitive amplified polymorphism,MSAP)技术分析了健康仿刺参(Apostichopus japonicus)体壁和“化皮病”仿刺参病变体壁、正常体壁DNA序列中CCGG位点的甲基化情况。结果显示,健康仿刺参体壁和“化皮病”仿刺参病变体壁、正常体壁总甲基化水平分别为(18.60±5.61)%、(26.70±6.82)%和(19.53±3.34)%,其中全甲基化水平分别为(13.97±4.86)%、(20.08±5.26)%和(15.42±2.61)%,半甲基化水平分别为(4.63±3.59)%、(6.62±3.80)%和(4.11±2.08)%。“化皮病”仿刺参病变体壁总甲基化水平和全甲基化水平显著高于健康仿刺参体壁和“化皮病”仿刺参正常体壁(P<0.05),健康仿刺参体壁与“化皮病”仿刺参正常体壁总甲基化水平和全甲基化水平差异不显著(P>0.05);三者的半甲基化水平无显著差异(P>0.05)。因此,推测仿刺参体壁“化皮”与DNA甲基化有关。
The methylation-sensitive amplified polymorphism (MSAP) technique was used to analyze the relationship between the body wall of Apostichopus japonicus and the wall of the “ DNA sequence in the CCGG site methylation situation. The results showed that the total methylation levels of normal body wall were (18.60 ± 5.61)%, (26.70 ± 6.82)% and (19.53 ± 3.34)%. The levels of methylation were (13.97 ± 4.86)%, (20.08 ± 5.26)% and (15.42 ± 2.61)% respectively, and the methylation levels were (4.63 ± 3.59)% and ± 3.80)% and (4.11 ± 2.08)%. The total methylation level and total methylation level of parietal gland in the ”Epidermis disease“ were significantly higher than those in the normal body wall of A. glabra and the normal body wall of ”Epidermis disease“ (P < 0.05). There was no significant difference in the total methylation level and total methylation level between the normal body wall and the normal body wall of ’ No significant difference (P> 0.05). Therefore, the speculation that the somatosensory wall ”skin" and DNA methylation.