目的　建立谷氨酸诱导的大鼠星形胶质细胞肿胀模型，观察 Ｄｙｎ Ａ１ ～１３ 对谷氨酸诱导的大鼠星形胶质细胞肿胀的影响。方法　［３ Ｈ］ ３氧甲基 Ｄ葡萄糖摄取测定细胞含水量。结果　①给予０５ ，１０ ，１００ ｍ ｍｏｌ· Ｌ－ １ 的谷氨酸作用１ ｈ 均可引起细胞的含水量增加。②强啡肽 Ａ（ Ｄｙｎ Ａ） 可降低谷氨酸诱导肿胀的大鼠星形胶质细胞含水量。③κ受体拮抗剂ｎｏｒ Ｂ Ｎ Ｉ可阻断 Ｄｙｎ Ａ１ ～１３ 降低谷氨酸诱导的肿胀大鼠星形胶质细胞含水量的作用。结论　谷氨酸可诱导大鼠星形胶质细胞肿胀； Ｄｙｎ Ａ１ ～１３ 可通过激活κ受体抑制谷氨酸诱导的大鼠星形胶质细胞肿胀 Objective To establish glutamate-induced rat astrocyte swelling model and observe the effect of Dyn A1-13 on glutamate-induced astrocyte swelling. Methods [3 H] 3 O methyl glucose uptake determination of cellular moisture content. RESULTS ① Administration of glutamate at 05, 1  0 and 100 mol · L -1 for 1 h all caused an increase in cell water content. Dynorphin A (Dyn A) can reduce glutamate-induced swelling of rat astrocyte water content. ③ κB antagonist nor  B N I can block Dyn A1 ~ 13 reduce glutamate-induced swelling of rat astrocyte water content. Conclusion Glutamic acid induces rat astrocyte swelling. Dyn A1-13 can inhibit glutamate-induced rat astrocyte swelling by activating kappa receptor