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本文研究的主要目的是利用微量组织培养的快速免疫荧光定量技术,以检测组织培养中的病毒,以及用快速免疫荧光法,在披盖病毒尚未复制的组织培养中检测该病毒抗原的存在。作者将Si、SF和WN病毒在BHK细胞内增殖,HC病毒则用PK-15细胞,BVD病毒采用小牛睾丸细胞。HC病毒感染的PK-15细胞须置于等量的磷酸缓冲盐水(PBS)中,以超声波击碎后,用超速离心法(20万g×30分钟)沉淀,上清液中含HC病毒的可溶性抗原。Si病毒、SF病毒、WN病毒和HC病
The main purpose of this study is to detect the virus in tissue culture by using the rapid immunofluorescence quantification technique of tissue culture and to detect the presence of the viral antigen in the tissue culture that has not yet been replicated by the rickety virus by rapid immunofluorescence. The authors propagate Si, SF and WN viruses in BHK cells, PK-15 cells for HC virus, and Bovine testicular cells for BVD virus. PK-15 cells infected with HC virus should be placed in an equal volume of phosphate buffered saline (PBS), disrupted by sonication, and precipitated by ultracentrifugation (200,000 g x 30 min). The supernatant containing HC virus Soluble antigen. Si virus, SF virus, WN virus and HC disease