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美国研究人员研制出专门针对人类免疫缺陷症病毒（HIV）感染细胞的融合蛋白TAT-Casp3,该蛋白由HIVTAT转导区和促细胞凋亡酶caspase3组成。研究人员用HIV蛋白酶裂解位点（可使融合蛋白被HIV蛋白酶激活）替代内源性caspase3裂解位点。融合蛋白在感染细胞中被HIV蛋白酶加工成活性蛋白,引起感染细胞凋亡。为检测HIV蛋白酶对融合蛋白的作用,研究人员先用蛋白酶抑制剂ritonavir处理HIV感染细胞,然后给该细胞以融合蛋白,结果没有作用,因为融合蛋白需要HIV蛋白酶才能有效。
US researchers developed a fusion protein specifically targeting human immunodeficiency virus (HIV) infected cells TAT-Casp3, the protein consists of HIVTAT transduction region and the pro-apoptotic enzyme caspase3. The researchers replaced the endogenous caspase 3 cleavage site with the HIV protease cleavage site (which allows the fusion protein to be activated by HIV protease). The fusion protein is processed into active protein by HIV protease in infected cells, causing apoptosis in infected cells. To test the effect of HIV protease on the fusion protein, the researchers first treated the HIV-infected cells with the protease inhibitor ritonavir and then given the cell a fusion protein, which gave no effect because the fusion protein required HIV protease to be effective.