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Objective: This paper attempts to discuss the effects of surviving antisense RNA on doxorubicin-induced apoptosis in pancreatic cancer cell line PANC-1. Methods: A surviving antisense eukaryotic vector pcDNA3-SV Vas prepared in previous study was delivered into PANC-1 by electroperforation. Cell survival fraction and MTT assay were used to investigate the sensibility of transfected cells to doxorubicin. Apoptosis was detected by DNA gel electrophoresis. Results: We obtained two positive cell clone PANC-1/SVVas and PANC-1/neo cells, the growth of PANC-1/SV Vas cells was significantly reduced (P＜0.05). By MTT assay, the IC50 to doxorubicin of PANC-1/SV Vas, PANC-1/neo and PANC-1 cells were (0.285±0.012) μmol/L, (1.528±0.317) μ mol/L and (1.540±0.253) μ mol/L respectively, the difference was significant by statistic analysis (P＜0.01). Agarose gel electrophoresis of genomic DNA from PANC/SV Vas showed typical DNA ladder, but DNA from PANC-1/neo and PANC-1 did not. Conclusion: Survivin antisense RNA could enhance doxorubicin-induced apoptosis in pancreatic cancer cell line PANC-1. This may lay an experimental foundation for further research of gene therapy in pancreatic cancer.