在农业上,正确鉴别植物寄生线虫和昆虫致病线虫有着重要的意义。以往常用的方法是形态学的分析,但这要求研究者具有较高的技能和丰富的经验。限制性片段长度多态性分析(RFLP)、PCR技术虽能清楚地分辨种间、种内差异,但存在着耗时、需大量DNA或结果不够准确的缺点。一般认为具有高度重复性的卫星DNA不具备什么功能, In agriculture, the correct identification of plant parasitic nematodes and insect pathogenic nematodes is of great significance. The most commonly used method is morphological analysis, but this requires researchers with high skills and rich experience. Restriction Fragment Length Polymorphism Analysis (RFLP), PCR technology can clearly distinguish interspecific and intraspecific differences, but there is a shortcoming of time-consuming, large amount of DNA or inaccurate results. It is generally considered that highly repetitive satellite DNA does not have any function,