论文部分内容阅读
本文采用单因素比较、正交设计法以及均匀设计法,分别考察影响绞股蓝愈伤组织诱导继代培养及其皂甙含量的因素。试验表明:MS基本培养基有利子其愈伤组织诱导。N_6、B_5基本培养基、低浓度的细胞分裂素、高浓度的NAA、黄豆粉、高浓度的蔗糖有利于绞股蓝愈伤组织的皂甙积累和鲜重增加。
In this paper, single factor comparison, orthogonal design and uniform design method were used to investigate the factors affecting the induction of subculture of Gynostemma pentaphyllum and its saponin content. The experiment showed that: MS basal medium favored its callus induction. The basic medium of N_6 and B_5, low concentration of cytokinin, high concentration of NAA, soybean flour and high concentration of sucrose were beneficial to the accumulation of saponin and the fresh weight of Gynostemma callus.