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目的 :探讨益气补肾药方延缓衰老的免疫学机制。方法 :以老龄小鼠为衰老模型 ,用凝胶迁移率 (EMSA)法 ,观察益气、补肾和益气补肾药方对抗CD3抗体诱导的老龄小鼠T细胞中NF κB活性的影响。用Westernblot分析该药方对NF κB两个亚基 (p65和p50 )的表达。结果 :老龄小鼠T细胞中NF κB的活性低于幼龄小鼠 ,3种药方均可不同程度地提高老龄小鼠T细胞中NF κB的活性 ;而对p65和p50的表达没有明显影响。结论 :益气补肾药方可通过提高T细胞中NF κB的活性 ,改善因衰老而导致的免疫功能紊乱。
Objective : To explore the immunological mechanism of Yiqi Bushen Prescription in delaying senility. METHODS : The effect of Yiqi, Bushen and Yiqi Bushen Prescription on the activity of NF κB in aged T cells induced by CD3 antibody was observed by using old age mouse as the aging model and gel mobility (EMSA) method. Western blot was used to analyze the expression of two subunits (p65 and p50) of NF κB. Results: The activity of NF κB in old mouse T cells was lower than that of young mice. All three prescriptions could increase the activity of NF κB in T cells of aged mice to varying degrees, but had no significant effect on the expression of p65 and p50. Conclusion: Yiqi Bushen Prescription can improve the immune function disorder caused by aging by increasing the activity of NFκB in T cells.