cAMP Modulates Macrophage Development by Suppressing M-CSF-Induced MAPKs Activation

来源 :中国免疫学杂志(英文版) | 被引量 : 0次 | 上传用户:Nuangfeng0915
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
M-CSF is a key cytokine in macrophage development by inducing MAPKs activation, and cAMP can inhibit MAPKs activation induced by inflammatory stimuli. To explore the effects of cAMP on M-CSF-induced MAPKs activation and on macrophage development, the model of bone marrow-derived murine macrophages (BMMs) was used. The effects of cAMP on M-CSF-induced MAPKs activation were analyzed by West blotting assay, and the effects of cAMP on CD14 and F4/80 expression during macrophage development were examined by FACS analysis.Macrophage morphology showed the successful establishment of the model of macrophage development. West blotting assay revealed that M-CSF activated ERK, JNK and p38 in both mature and immature macrophages, and cAMP inhibited M-CSF-induced ERK, JNK and p38 activation in a time-dependent manner. FACS analysis revealed that macrophage development was impaired with cAMP pretreatment. In conclusion, cAMP modulates macrophage development by suppressing M-CSF-induced MAPKs activation.
其他文献
目的:比较不同预处理异基因造血干细胞移植(allo-HSCT)后早期树突状细胞(DCs)亚群重建情况,以及移植物中CD34+细胞是否影响移植后早期DCs亚群重建.方法:采用三色流式细胞仪动
【摘 要】目的:观察托拉塞米治疗颅脑创伤后脑水肿的疗效。方法:将186例颅脑创伤患者随机分为两组,对照组93例给予呋塞米治疗,治疗组93例给予托拉塞米治疗,两组均联合甘露醇125ml静点,q6h,第8天开始依据病情变化调整剂量,两组疗程均为14天,重症者给予心电监护、吸氧等,同时给予常规营养支持及对症治疗,记录每小时及24小时尿量。并通过监测颅内压、电解质水平,观察用药前后颅内压的变化、观察用药前
期刊
In vivo studies showed that dendritic cell (DC) dysfunction occurred in tumor microcnvironment. As tumors were composed of many kinds of cells, the direct effec
A key and limiting step in the process of human monocyte-derived dendritic cells (mDCs) for clinical use is their in vitro maturation and in vivo migration. We
CD59 may inhibit the cytolytic activity of complement by binding to C8/C9 and protect host cell membranes against homologous membrane attack complex (MAC). Howe
秋季腹泻又叫轮状病毒肠炎,多发生在6~24个月的婴幼儿,多伴有发热和上呼吸道感染症状,呕吐,大便次数多、量多,常伴有脱水和酸中毒。对我院2009年5—11月门诊及住院秋季腹泻患
期刊
目的 探讨重症监护病房(ICU)呼吸道病原菌种类及耐药性分析,为防治院内感染提供依据.方法 对我院2007年1月-2008年12月分离出的368株ICU呼吸道感染病原菌分布及耐药性分析.药
IL-12 priming plays an important role in stimulation of CD8+ effector T cells and development of CD8+ memory T (Tm) cells. However, the functional alteration of
【摘 要】柴胡和肝胶囊是我院在40多年临床工作中研制出来的治疗慢性肝炎的中成药。将我院慢性肝炎患者76例随机分为两组,治疗组治疗后透明質酸、Ⅳ型胶原与治疗前比较明显改善(p<0.05)。柴胡和肝胶囊有活血化瘀、软坚散结,抗肝纤维化的作用。  【关键词】柴胡和肝;慢性肝炎;疗效观察  【中图分类号】R512.62 【文献标识码】A 【文章编号】1008-6455(2012)01-03
期刊