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目的探讨华支睾吸虫病患者可溶性血管内皮细胞黏附分子(sVCAM-1)的表达,以及与肝功能的临床关系。方法50例未经治疗的华支睾吸虫病患者,参照Child-Pugh肝功能分级法,分成A22例、B 14例、C 14例3组。采用双抗体夹心ELISA法,检测血清sVCAM-1水平;采用放射免疫分析方法检测血清白细胞介素-4(IL-4)含量;采用鲎三肽基质染色定量法检测血清细菌脂多糖(LPS)水平。对照组为健康献血员20例。结果(1)华支睾吸虫病患者血清sVCAM-1、LPS和IL-4的水平明显高于对照组;(2)经相关性检验,患者血清sVCAM-1、LPS含量与TBL、ALT均呈正相关,与ALB呈负相关;(3)参照Child-Pugh分级,肝功能受累越重,患者血清中sVCAM-1、LPS和IL-4含量依次递增。结论sVCAM-1及LPS参与介导了华支睾吸虫病肝损伤过程;IL-4对华支睾吸虫感染具有一定的保护作用。
Objective To investigate the expression of soluble vascular endothelial cell adhesion molecule (sVCAM-1) in Clonorchis sinensis and its clinical relationship with liver function. Methods Fifty untreated patients with clonorchiasis were divided into A22, B 14 and C 14 in 3 groups according to Child-Pugh liver function classification. Serum levels of sVCAM-1 were detected by double antibody sandwich ELISA. Serum interleukin-4 (IL-4) levels were measured by radioimmunoassay. Serum bacterial lipopolysaccharide (LPS) . Control group of healthy blood donors in 20 cases. Results (1) Serum levels of sVCAM-1, LPS and IL-4 in patients with Clonorchiasis sinensis were significantly higher than those in control group. (2) Serum levels of sVCAM-1 and LPS were positively correlated with TBL and ALT (3) According to the Child-Pugh classification, the more serious the liver function was, the contents of sVCAM-1, LPS and IL-4 in the serum increased in turn. Conclusions sVCAM-1 and LPS are involved in the process of hepatic injury induced by Clonorchis sinensis. IL-4 may have a protective effect on Clonorchis sinensis infection.