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目的 观察间皮细胞在培养状态下的形态学特征 ,测定其抗凝与纤溶作用的强度 ,为血管假体内腔铺被细胞的选择提供依据。 方法 取SD大鼠大网膜、主动脉、皮下结缔组织用作间皮、内皮及成纤维细胞的培养。倒置显微镜及扫描电镜下观察各自的形态特点。取 3类细胞第 3代融合后 4 8h的无血清培养液 ,放射免疫法测定 6 酮 PGF1α(前列环素的代谢产物 )的含量 ,发色底物法测定纤溶酶原激活物活性。 结果 在相同培养状态下 ,间皮细胞生长较快 ,表现出许多与内皮细胞相似的形态特点。间皮细胞培养液中 6 酮 PGF1α浓度显著高于成纤维细胞及内皮细胞培养液中的浓度 ,纤溶酶原激活物活性也高于成纤维细胞者 ,但与内皮细胞相比无显著性差异。结论 在相同培养状态下 ,间皮细胞具有与内皮细胞相似的结构与功能特点 ,可能为血管假体内腔铺被层的理想材料。
Objective To observe the morphological characteristics of mesothelial cells under culture conditions and determine the strength of anticoagulation and fibrinolysis to provide the basis for the choice of the cells to be coated by vascular prostheses. Methods The omentum, aorta and subepithelial connective tissues of SD rats were used to culture mesothelial, endothelium and fibroblasts. Inverted microscope and scanning electron microscope to observe their morphological characteristics. Serum-free medium of 48h after the 3rd generation fusion of the 3 types of cells was taken out. The content of 6-keto PGF1α (the metabolite of prostacyclin) was determined by radioimmunoassay. The activity of plasminogen activator was determined by chromogenic substrate method. Results Under the same culture conditions, mesothelial cells grew rapidly and showed many morphological features similar to those of endothelial cells. The concentration of 6-keto-PGF1α in mesothelial cells was significantly higher than that in fibroblasts and endothelial cells, and the activity of plasminogen activator was also higher than fibroblasts, but no significant difference compared with endothelial cells . Conclusion Under the same culture condition, mesothelial cells have the same structural and functional characteristics as endothelial cells, which may be the ideal material for vascular prosthetic layer lining.