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F-box蛋白参与细胞周期调控、细胞凋亡及信号转导等多种生命活动,对维持植物正常生长发育a和介导非生物胁迫响应等过程发挥重要作用。谷子具有显著的耐旱耐瘠薄等特性,本研究根据谷子转录组分析结果,从525个F-box家族成员中鉴定出19个在干旱胁迫下表达量上调的F-box基因,根据序列相似性将其分为6类,同一类基因具有相似的内含子-外显子结构。染色体定位分析发现,这些基因分别分布在谷子的8条染色体上,其中,第2条染色体上含F-box基因最多,有6个。结构域分析结果表明,19个F-box蛋白均含保守的F-box结构域,而C端含FBD、WD40、FBA、ZnF、Kelch和LRR等结构域。启动子元件分析表明,谷子19个F-box基因均含逆境应答元件,其中,MYB和MYC元件的数量最多(9~78个),说明这些基因对干旱应答反应可能主要受MYB、MYC转录因子调控。转录组分析结果表明,SiF-box18基因对干旱胁迫的响应远远高于其他F-box成员,对干旱、高盐、ABA、SA和JA都有响应;亚细胞定位结果显示,SiF-box18蛋白定位在细胞核中。本研究为进一步深入了解SiF-box18基因的功能提供了依据。
F-box proteins are involved in a variety of life activities such as cell cycle regulation, apoptosis and signal transduction and play an important role in maintaining normal plant growth and development and in mediating response to abiotic stress. In this study, based on the analysis of the transcriptome of millet, we identified 19 F-box genes with up-regulated expression under drought stress from 525 F-box family members. According to the sequence similarity Divided into six categories, the same type of gene has a similar intron - exon structure. Chromosome localization analysis found that these genes were distributed in the eight chromosomes of the millet, of which, the first two chromosomes contain F-box genes up to six. The results of domain analysis showed that all the 19 F-box proteins contained a conserved F-box domain and the C-terminal domain contained FBD, WD40, FBA, ZnF, Kelch and LRR. The analysis of promoter elements showed that all of the 19 F-box genes in millet contained stress response elements, of which the MYB and MYC elements were most abundant (9-78), suggesting that the responses of these genes to drought may be mainly affected by MYB, MYC transcription factor Regulation. Transcriptome analysis indicated that the response of SiF-box18 to drought stress was much higher than that of other F-box members, and responded to drought, high salt, ABA, SA and JA. The results of subcellular localization showed that SiF-box18 Positioning in the nucleus. This study provides a basis for further understanding of the function of SiF-box18 gene.