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为研究土壤盆栽和溶液培养的‘84K’(Populus alba×P.glandulosa)离体根系的根压及根压昼夜节律,系统探讨影响根压的因素及它们与根压的关系,深入了解根压的产生机制及其节律调控,采用压力转换器法对‘84K’离体根系的根压进行了研究。该研究采用不同时间取样、摘叶、环割等试验处理,不同土壤温度、昼夜温差/恒温等测定条件研究了离体根压的节律,并进一步运用化学抑制剂研究根系呼吸代谢、根系导水率对根压的影响,并测定伤流液的渗透强度及其无机离子和可溶性糖含量日变化以探讨它们和根压昼夜节律的关系。研究发现,‘84K’离体根系的根压具有昼高夜低的周期节律,在上午至正午达到峰值,在20:00达到谷值。不同取样时间、不同培养介质对根压周期性有一定的影响,但总体昼高夜低没有改变。摘叶、环割、呼吸抑制剂或细胞膜水导抑制剂影响根压峰值的大小,但对根压的昼夜节律没有明显影响。摘叶、环割或呼吸抑制剂显著地降低了根压峰值,而水导抑制剂对根压峰值影响不大,说明根压峰值的大小主要受呼吸作用,包括呼吸底物的影响,而根系水导率对根压峰值的影响不大。根压峰值随土壤温度下降而下降、土壤温度还改变根压的昼夜节律。随温度的变化,最大根压和呼吸速率同步变化,意味着根压随温度的变化也是呼吸代谢在起作用。根系伤流液渗透强度白天高晚上低。伤流液无机离子含量以及可溶性糖含量的日变化和渗透强度变化一致,其中无机离子扮演更主要的角色。昼夜温差条件下的根压峰值显著大于昼夜常温条件下的根压峰值。总之,‘84K’根压呈现较稳定的昼夜周期性,昼高夜低。根压峰值大小主要受呼吸代谢的调控,呼吸抑制剂、呼吸代谢底物、影响呼吸作用的温度等都影响‘84K’根压峰值的大小。根系导水率对根压有一定的影响,但不显著。
In order to study the root pressure and root pressure circadian rhythm of ’84K’ (Populus alba × P.glandulosa) cultured in potted soil and solution culture, the factors influencing root pressure and their relationship with root pressure were systematically investigated to understand root pressure The mechanism of root production and its rhythm regulation were studied. The root pressure of ’84K’ root system was studied by pressure transducer method. In this study, the root-mean-square root-mean-square root-mean-square (RMS) rhythm was studied by sampling at different times, leaf picking, ring mowing and other test treatments, different soil temperatures, diurnal temperature / Rate on the root pressure, and determine the infiltration of wound fluid and inorganic ions and soluble sugar content changes to explore their relationship with the root pressure circadian rhythm. The study found that the roots pressure of ’84K’ ex-rooted root system had the circadian rhythm of daytime and night, reaching the peak value between noon and noon and reaching the trough at 20:00. Different sampling time, different culture medium on the root pressure cyclical have a certain impact, but the overall day and night no change. Abstract leaves, ring cut, respiratory inhibitors or cell membrane conductivity inhibitors affect the size of the root pressure peak, but have no significant effect on the circadian rhythm of root pressure. Abstract leaves, ring cut or respiratory inhibitors significantly reduced the root pressure peak, while water-based inhibitors had little effect on the root pressure peak, indicating that the root pressure peak is mainly due to respiration, including respiratory substrates, while roots Water conductivity has little effect on the root pressure peak. The root pressure peak value decreased with the decrease of soil temperature, soil temperature also changed the circadian rhythm of root pressure. With the temperature changes, the maximum root pressure and respiratory rate changes simultaneously, which means that changes in root pressure with temperature is also respiratory metabolism at work. Root bleeding fluid penetration intensity during the day and night low. The changes of inorganic ion content and soluble sugar content in wound fluid were consistent with the changes of infiltration intensity, in which inorganic ions played a more important role. The peak pressure of root pressure under the temperature difference between day and night was significantly higher than that under day and night temperature. In short, ’84K’ root pressure showed a more stable circadian cycle, day and night at low. Root pressure peak size is mainly controlled by respiratory metabolism, respiratory inhibitors, respiratory metabolism substrates, the impact of respiratory temperature and so affect the ’84K’ root pressure peak size. Root hydraulic conductivity had a certain impact on root pressure, but not significant.