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目的:研究凝结芽胞杆茵(Bacillus coagulans,BC)对右旋葡聚糖硫酸钠(DSS)引发的大鼠溃疡性结肠炎(UC)的治疗作用.方法:采用DSS引发大鼠UC,分别用10~7和10~6 CFU/mL BC、0.02g/mL柳氮磺胺吡啶(SASP)和生理盐水(NS)处理.21d后测定动物体质量、结直肠湿重、肠黏膜溃疡、糜烂点数及面积、髓过氧化物酶活性和肠道菌群培养.结果:治疗21d后,各组中大鼠的体质量增加,以BC10~7 CFU/mL增重明显(P<0.05);各治疗组中大鼠的结直肠湿重、肠溃疡糜烂点数、面积、MPO活性与模型组比较均有显著性差异(P<0.05或P<0.01或P<0.001);肠道菌群分析,造型后双歧杆菌数明显下降(P<0.05),治疗后各组的双歧杆菌数量均明显增加(P<0.01或P<0.001),以BC组和SASP组增加最明显(P<0.01或P<0.001).BC在肠道定植.结论:BC对DSS引发的大鼠UC有明显治疗作用.
OBJECTIVE: To study the therapeutic effect of Bacillus coagulans (BC) on dextran sodium sulfate (DSS) -induced ulcerative colitis (UC) in rats.Methods: UC rats were induced by DSS, 10 ~ 7 and 10 ~ 6 CFU / mL BC, 0.02g / mL sulfasalazine (SASP) and normal saline (NS) .The body weight, colorectal wet weight, intestinal mucosal ulcer, Area, myeloperoxidase activity and intestinal microflora.Results: After 21 days of treatment, the body weight of rats in each group increased significantly (P <0.05) with BC10 ~ 7 CFU / mL, There was significant difference between the model group and the wet weight of colorectal mucosa (P0.05 or P0.01 or P0.001) in colorectal wet weight, intestine erosion point, area and MPO activity in rats, The number of Bifidobacterium significantly decreased (P <0.05). After treatment, the number of Bifidobacteria increased significantly in each group (P <0.01 or P <0.001), and increased most significantly in BC and SASP groups (P <0.01 or P <0.001) ) .BC was colonized in the intestine.Conclusion: BC has a significant therapeutic effect on DSS-induced UC in rats.